Thermo Fisher Scientific F(ab)-Goat anti-Mouse IgG1 Fc Secondary Antibody, Alexa Fluor 647
Alexa Fluor 647로 결합된 염소 유래 F(ab) 항-마우스 IgG1 Fc 2차 항체로, 낮은 비특이적 결합과 우수한 조직 침투력을 제공. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 650/671 nm의 형광 특성을 가짐.
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Applications and Recommended Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–2 µg/mL |
| Peptide Array (Array) | 250 ng/well |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG1 Fc |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient |
| RRID | AB_3099343 |
| Target | IgG Fc |
| Antibody Form | F(ab) Fragment |
Available Formats
- Alexa Fluor 405
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 700
- Custom conjugation available upon request
Product Specific Information
This F(ab)-Goat anti-Mouse IgG1 Fc specific antibody is a goat polyclonal F(ab) fragment that specifically binds to the Fc region of mouse IgG1 isotype. It shows minimal to no cross-reactivity with mouse IgG2a, IgG2b, IgG3, and other species (rat, rabbit, guinea pig, bovine, hamster, horse, sheep, human, monkey, donkey, chicken IgG). Mild cross-reactivity with rat IgG2a may occur.
F(ab) fragments offer advantages over whole IgG antibodies due to their smaller size (~50 kDa vs. ~150 kDa) and lack of Fc region:
- Improved tissue penetration and staining in IHC
- Reduced nonspecific background from Fc receptor binding
- Enhanced multiplexing capability in ICC, IHC, Flow Cytometry, and Western Blot
Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to minimize nonspecific background caused by aggregates.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies designed for specific detection of mouse immunoglobulins. These antibodies are widely used in detection, sorting, and purification applications.
They enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody. Typically generated by immunizing the host with pooled mouse immunoglobulins, they can be further purified or modified (e.g., by immunoaffinity chromatography, fragmentation, or conjugation) for specific research needs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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