Thermo Fisher Scientific CD3 Monoclonal Antibody (1F4)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:25

-

Flow Cytometry (Flow)

1:10-1:25

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Rat

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

1F4

Immunogen

F344 rat T cells stimulated with PMA (TPA) and calcium ionophore. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD3,uniProtId:P19377-1,ncbiNodeId:10114,antigenRange:1-173,antigenLength:173,antigenImageFileName:MA1-80469_CD3_P19377-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80469_CD3_P19377-1_House_mouse_PDP.jpeg,sortOrder:11},{targetFamily:CD3,uniProtId:Q64159-1,ncbiNodeId:10114,antigenRange:1-182,antigenLength:182,antigenImageFileName:MA1-80469_CD3_Q64159-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80469_CD3_Q64159-1_House_mouse_PDP.jpeg,sortOrder:12}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928106

Product Specific Information

This clone is suitable for use on paraffin embedded material using an antigen-unmasking fluid. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a approximately 25 kDa antigen which is found on rat T-cells.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.