Thermo Fisher Scientific CD3 Monoclonal Antibody (1F4)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This clone is suitable for use on paraffin-embedded material using an antigen-unmasking fluid.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.

Mouse anti-Rat CD3 antibody, clone 1F4, recognizes rat CD3, an approximately 25 kDa antigen found on rat T-cells.

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits (CD3γ, CD3δ, CD3ε, and CD3ζ). These subunits are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.

CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated by a double tyrosine-based motif present in the CD3 subunits.
CD3 may play a role in TCR-induced growth arrest, cell survival, and proliferation.

The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum. It is not expressed on B or NK cells.
Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
The genes encoding the CD3ε, CD3γ, and CD3δ polypeptides are located on chromosome 11.
Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.