Thermo Fisher Scientific NeuN Recombinant Mouse Monoclonal Antibody (PD01-45)
NeuN 단백질을 인식하는 재조합 마우스 단클론 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot 및 면역조직화학(IHC) 분석에 적합하며, 34 kDa 밴드를 검출합니다. 세포질 및 핵에 존재하는 Fox-3(RBFOX3) 단백질 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD01-45 |
| Immunogen | Synthetic peptide within human NeuN aa 20–60/312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3093737 |
Product Specific Information
- Positive control: SH-SY5Y cell lysate, SHG44 cell lysate, human brain tissue, mouse hippocampus, brain, cerebellum tissues, rat hippocampus, brain, cerebellum tissues, mouse cerebral cortex tissue.
- Predicted band size: 34 kDa
- Subcellular Location: Cytoplasm, Nucleus
Target Information
Fox-3, also known as NeuN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein containing one RRM (RNA recognition motif) domain. It localizes to both the nucleus and cytoplasm and is suggested to regulate alternative splicing events.
Fox-3 is encoded by a gene on human chromosome 17, which comprises over 2.5% of the human genome and encodes more than 1,200 genes. Two key tumor suppressor genes on this chromosome, p53 and BRCA1, are essential for maintaining genetic integrity. p53 regulates DNA repair and apoptosis, while BRCA1 is involved in DNA repair and is linked to early-onset breast and ovarian cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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