Thermo Fisher Scientific NeuN Recombinant Mouse Monoclonal Antibody (PD01-45)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PD01-45

Immunogen

Synthetic peptide within human NeuN aa 20-60/312. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NeuN,uniProtId:A6NFN3-1,ncbiNodeId:9606,antigenRange:20-60,antigenLength:312,antigenImageFileName:MA5-50648_NeuN_A6NFN3-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50648_NeuN_A6NFN3-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093737

Product Specific Information

Positive control: SH-SY5Y cell lysate, SHG44 cell lysate, Human brain tissue, mouse hippocampus tissue, mouse brain tissue, mouse cerebellum tissue, rat hippocampus tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue lysates, mouse cerebral cortex tissue.

Predicted band size: 34 kDa

Subcellular Location: Cytoplasm, Nucleus.

Target Information

Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein that contains one RRM (RNA recognition motif) domain. Localized to both the nucleus and cytoplasm, Fox-3 is suggested to regulate alternative splicing events. Fox-3 is encoded by a gene located on human chromosome 17, which is comprised over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and LiFraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.