Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), Super Bright 436, eBioscience

Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), Super Bright 436, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg/test)
• Publications: View 3 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high-affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (in 100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test.

Super Bright 436 can be excited with the violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD64 (FCGR1A) encodes a high-affinity Fc-gamma receptor involved in immune response. It is one of three related gene family members on chromosome 1. Dysfunction of CD64 has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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