Thermo Fisher Scientific CCL4 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN.

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1beta (CCL4). Anti-Murine MIP-1beta (CCL4)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect Murine MIP-1beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-1beta (500-P213) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine MIP-1beta.

Western Blot
To detect Murine MIP-1beta by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine MIP-1beta is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information
500-P213BT-1MG will be provided as 2 × 500 µg.

Target Information

CCL4 (Macrophage Inflammatory Protein 1-beta, MIP-1β)
CCL4 belongs to the intercrine beta (chemokine CC) family and plays roles in chemotactic and proinflammatory responses, as well as homeostasis. It is produced by macrophages upon stimulation by bacterial endotoxins and recruits various inflammatory cells to sites of inflammation.

CCL4 is secreted by lymphocytes, macrophages, and dendritic cells. Both CCL4 and CCL3 regulate the trafficking and activation of inflammatory cells in response to bacterial, viral, parasitic, and fungal infections. While both act on monocytes, CCL4 selectively attracts CD4+ lymphocytes, whereas CCL3 attracts CD8+ lymphocytes. They are also potent chemoattractants for B cells, eosinophils, and dendritic cells.

The processed form of CCL4 can down-regulate surface expression of the CCR5 receptor, thereby inhibiting CCR5-mediated HIV-1 entry into T cells. CCL4 binds with high affinity to CCR5 receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.