Thermo Fisher Scientific POLR3A Polyclonal Antibody

Applications

• Western Blot (WB): 0.2–1 µg/mL

Product Specifications

Product Specific Information

• Peptide sequence: AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT
• Sequence homology:
  Cow: 79%; Dog: 86%; Guinea Pig: 86%; Horse: 79%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 86%; Yeast: 85%

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III, which synthesizes small RNAs such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. The single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site, promoting translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching between straight and bent conformations during nucleotide addition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.