
Thermo Fisher Scientific Goat anti-Human IgE Cross-Adsorbed Secondary Antibody, HRP
Thermo Fisher Scientific의 Goat anti-Human IgE HRP 결합 2차 항체로, 인간 IgE 검출용. WB, IHC, ELISA에 적용 가능. 교차흡착 처리로 비특이적 반응 최소화. 고순도 친화 크로마토그래피 정제 및 안정적 HRP 결합.
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Thermo Fisher Scientific Goat anti-Human IgE Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgE |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535576 |
| Target | IgE |
| Cross Adsorption | Against bovine, mouse, and rabbit serum proteins |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- AP
- Biotin
- FITC
- TRITC
- Custom conjugation available upon request
Product Specific Information
Rehydrate with 1.1 mL of deionized water and allow to stand for 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove particulates. Prepare fresh working dilutions daily. Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, or serum proteins from bovine, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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