Thermo Fisher Scientific CD146 Recombinant Rabbit Monoclonal Antibody (5)
Human CD146 단백질을 표적으로 하는 재조합 토끼 모노클로날 항체. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 보존제가 없는 고순도 단백질 A 정제 제품으로 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5 |
| Immunogen | Recombinant Human CD146 protein (Met1–Gly559) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785279 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human CD146/MCAM.
Target Information
CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and other normal and malignant cell types. CD146 functions as a calcium-independent cell adhesion molecule and may act as a signal transduction molecule through Fyn kinase recruitment and tyrosine phosphorylation of intracellular proteins involved in actin cytoskeleton assembly.
Biological roles of CD146:
- Cell adhesion and endothelial monolayer cohesion
- Interaction with vascular elements enhancing tumor spread
- Possible role in neural crest cell adhesion during embryonic development
- Acts as a surface receptor triggering FYN and PTK2 phosphorylation and transient calcium increase
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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