Thermo Fisher Scientific CD146 Recombinant Rabbit Monoclonal Antibody (44)
Human CD146 단백질을 인식하는 재조합 토끼 단일클론 항체로 높은 특이성과 민감도를 제공. WB, IHC, ICC, Flow, ELISA 등 다양한 실험에 최적화된 희석 범위 적용 가능. 방부제 무첨가, 일관된 로트 간 품질 유지. 연구용으로만 사용.
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Thermo Fisher Scientific CD146 Recombinant Rabbit Monoclonal Antibody (Clone 44)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 44 |
| Immunogen | Recombinant Human CD146 protein (Met1–Gly559) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785281 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human CD146/MCAM.
Target Information
CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and other normal and malignant cell types. CD146 functions as a calcium-independent cell adhesion molecule and may also act as a signal transduction molecule recruiting Fyn kinase, leading to tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly.
CD146 plays a role in:
- Cell adhesion and cohesion of endothelial monolayers
- Interaction of melanoma cells with vascular elements, enhancing metastasis
- Neural crest cell adhesion during embryonic development
- Surface receptor-mediated tyrosine phosphorylation of FYN and PTK2, with transient intracellular calcium increase
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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