Thermo Fisher Scientific MBD2 Recombinant Rabbit Monoclonal Antibody (JE57-73)
MBD2 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, Flow Cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, HEK293 세포에서 발현된 IgG 형 항체입니다. 단백질 A로 정제된 액상 형태로, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE57-73 |
| Immunogen | Recombinant protein within human MBD2 aa 50–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911661 |
Product Specific Information
- Positive Control: Raji cell lysates, rat kidney tissue, human lung carcinoma tissue, human breast carcinoma tissue, Daudi
- Subcellular Location: Nucleus
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, which act as heterochromatic adaptor molecules involved in gene silencing and chromatin structure. High-affinity recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain, serving as a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double primary mouse fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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