Thermo Fisher Scientific Phospho-FAK (Tyr407) Polyclonal Antibody
FAK 단백질의 Tyr407 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용 가능. 고순도 Protein A 정제, 액상 형태로 안정적 보관 가능.
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- pk
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Thermo Fisher Scientific Phospho-FAK (Tyr407) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human FAK around the phosphorylation site of Tyr407 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. It localizes to focal adhesions, sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.
Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, leading to activation of ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, association of v-Src, Grb2, and Sos with FAK is independent of fibronectin adhesion. The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925. Several transcript variants encoding different isoforms have been identified for the FAK gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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