
Thermo Fisher Scientific Human STK25 (YSK1), GST Tag Recombinant Protein
인간 유래 STK25(YSK1) 단백질로 GST 태그가 부착된 재조합 단백질입니다. MAP kinase 신호 전달 연구용으로 적합하며, 인산화 효소 활성 분석에 활용됩니다. -80°C에서 안정적으로 보관 가능하며, 고순도 정제된 액상 형태로 제공됩니다.
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Thermo Fisher Scientific Human STK25 (YSK1), GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution: Assay-dependent
Publications: N/A
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | Full length |
| Tag | GST-tag |
| Molecular Weight | 75.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris (pH 7.5), 0.5 mM EDTA, 2 mM DTT, 50% glycerol, 0.02% Triton X-100, 150 mM NaCl |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- For maximum recovery, please spin prior to use.
- Aliquots of 5 µg, 10 µg, and 20 µg kinase sizes are not recommended; use original packaging until exhausted.
- For larger sizes, minimize freeze/thaw cycles by preparing aliquots (not below 20 µL).
- Never store diluted kinase.
- Properly stored at -80°C, product guaranteed for 6 months from purchase date.
Protein Form: Full Length
Target Information
STK25 (YSK1) is a serine/threonine kinase belonging to the GCK family. It acts as an upstream regulator of MAP kinase signaling pathways. Protein kinases transfer a phosphate group from ATP to substrate proteins, mediating signal transduction in eukaryotic cells.
They regulate metabolism, transcription, cell cycle, cytoskeletal rearrangement, movement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products, classified into 8 major groups based on catalytic domain sequence.
The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of kinases related to MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, Ste20/MAP4K).
MAP kinase cascades have been well characterized in the yeast pheromone response pathway, where pheromones activate MAPK signaling via Ste surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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