Thermo Fisher Scientific GITRL Polyclonal Antibody, PeproTech
Human GITRL 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 세포 증식, 분화, 생존 관련 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human AITRL |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930019 |
Product Specific Information
AA Sequence of recombinant protein:
METAKEPCMA KFGPLPSKWQ MASSEPPCVN KVSDWKLEIL QNGLYLIYGQ VAPNANYNDV APFEVRLYKN KDMIQTLTNK SKIQNVGGTY ELHVGDTIDL IFNSEHQVLK NNTYWGIILL ANPQFIS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human AITRL. Anti-Human AITRL-specific antibody was purified by affinity chromatography employing an immobilized Human AITRL matrix.
Sandwich ELISA:
To detect Human AITRL by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human AITRL (500-P244Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human AITRL.
Western Blot:
To detect Human AITRL by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human AITRL is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through activation of NF-kappa-B and various mitogen-activated protein kinases.
The glucocorticoid-induced tumor necrosis factor receptor (GITR) is expressed on CD4+ CD25+ regulatory T cells and plays a crucial role in immune regulation. Its ligand, GITRL, is expressed in endothelial and antigen-presenting cells, activating NF-kappa-B, inducing both pro- and anti-apoptotic effects, inhibiting regulatory T cell suppressive activity, and co-stimulating responder T cells through GITR.
Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappa-B activation, indicating that the GITRL-GITR pathway involves both NIK and TRAF2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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