Thermo Fisher Scientific eIF4E Polyclonal Antibody
Thermo Fisher Scientific의 eIF4E Polyclonal Antibody는 인간 및 생쥐 반응성을 가진 rabbit IgG 항체로, Western blot과 Immunoprecipitation에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human eukaryotic translation initiation factor 4E |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Bovine and Rabbit
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export.
All eukaryotic cellular mRNAs are blocked at their 5′ ends with the 7-methylguanosine cap structure (m7GpppX, where X is any nucleotide).
EIF4E directs ribosomes to the cap structure of mRNAs and exists as both a free form and as part of the multiprotein complex EIF4F.
EIF4E is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA–ribosome binding step of protein synthesis.
Other subunits of EIF4F include EIF4A (50 kD, ATPase and RNA helicase activities) and EIF4G (220 kD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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