Invitrogen™

Zero Blunt™ PCR Cloning Kit, without competent cells

The Zero Blunt™ PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with자세히 알아보기

The Zero Blunt™ PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.

Features of the Zero Blunt™ Cloning™ Kit with pCR™-Blunt vector:
• Fast & convenient—5-minute, room-temperature ligation
• Efficient—_ccd_B gene for positive selection results in >80% clones with correct insert
• Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection

The pCR™-Blunt vector provides:
• Eco_R I sites flanking the PCR product insertion site for excision of inserts
• T7 promoter/primer site for _in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening

How Zero Blunt™ PCR Cloning Works
The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector contains the lethal _E. coli__ccd_B gene fused to the C-terminus of _Lac_Zα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the _lac_Zα-_ccd_B gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.

Kit Configurations
The Zero Blunt™ PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent cells (K2750-20 and K2750-40) in 20- and 40- reaction kit sizes.

사양

박테리아 또는 효모 균주Not Included

클로닝 방법Blunt PCR

형식Kit

제품라인TOPO™, Zero Blunt™

제품 유형PCR Cloning Kit

프로모터T7

수량20 Reactions

벡터Blunt DNA Cloning Vectors

Unit SizeEach