Invitrogen™

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R Chemically Competent E. coli (Supply Center Only)

TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. TOPO™자세히 알아보기

TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. TOPO™ TA Cloning™ Kits use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. The newest TOPO™ TA Cloning™ Kit comes with a Gateway™ (GW) entry vector, pCR™8/GW/TOPO™ (Figure 1). This vector has:

• 3´-T overhangs for direct ligation of Taq_-amplified PCR products
• _att sites for rapid recombination into a variety of Gateway™ destination vectors
• Novel GW primer sites, within the att regions, located less than 55 base pairs from the PCR product insertion site for convenient sequencing
• Spectinomycin resistance gene for robust selection in E. coli
• _Eco_R I sites flanking the PCR product insertion site for easy excision of inserts

Selected TOPO™ TA Cloning™ Kits for entry into the Gateway™ Technology are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.

사양

클로닝 방법TOPO™ with Gateway™

반응 수20 reactions

제품라인One Shot™

제품 유형TA Cloning Kit

수량20 Reactions

배송 조건Dry Ice

벡터pCR

Unit SizeEach