Thermo Fisher Scientific RAD51 Monoclonal Antibody (RAD51, 2856)
RAD51 단백질을 표적으로 하는 Thermo Fisher Scientific의 마우스 모노클로날 항체. 인간 RAD51에 반응하며 IHC 및 Peptide Array에 적합. 단백질 A/G로 정제된 액상 항체로, 4°C에서 보관. DNA 복구 연구 및 RAD51 관련 질환 연구에 유용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RAD51, 2856 |
| Immunogen | Recombinant fragment of human RAD51 protein (around aa 1–134) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recombination.
It binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity.
RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners, forming a joint molecule between a processed DNA break and the repair template.
It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for homology search and strand exchange.
Mutations in the gene can result in breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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