Thermo Fisher Scientific PAPPA Monoclonal Antibody (PAPP A 1-24)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

PAPP A 1-24

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.04 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1086779

Product Specific Information

ABS 006-24-02 detects native Pregnancy-associated plasma protein A (PAPP-A) from human samples. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

ABS 006-24-02 has been successfully used in Western blotting, Immunohistochemistry and ELISA procedures. By Western blot, only unreduced forms of PAPA-A can be detected. In sandwich ELISA the optimal combination was BTE 004-09-02 as coating and biotinylated ABS 006-24-02 as detection. ABS 006-24-02 give the strongest singal in IHC compared to ABS 006-01-02 and BTE 004-09-02.

The epitope of ABS 006-24-02 is different from BTE 004-09-02 and ABS 006-01-02. The epitope is located on the PAPP-A subunit of the heterotetramer.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down`s syndrome (trisomy 21).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.