Thermo Fisher Scientific RAD51 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunocytochemistry (ICC/IF)

1:100-1:6,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified full-length recombinant Rad51 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_1960219

Product Specific Information

PA1-4985 detects Rad51 from human, mouse, and chicken samples.

PA1-4985 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and indirect immunofluorescence applications. By Western blot, this antibody detects a 37 kDa band representing Rad51 from Hela cells. GFP-tagging of Rad51 protein at either N- or C- terminus inactivates the function of Rad51 and therefore it cannot be used for the study of foci formation instead of the antibody.

The PA1-4985 immunogen is full-length recombinant Rap51 protein.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.