Thermo Fisher Scientific Glucocorticoid Receptor Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,500

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:250

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

-

View 4 publications 4 publications

Gel Shift (GS)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Reptile, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: A(150) P T E K E F P K T H S D V S S E Q Q H L K G Q T G(175) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Glucocorticoid Receptor (Nr3c1),uniProtId:P04150-1,ncbiNodeId:9606,antigenRange:150-175,antigenLength:777,antigenImageFileName:PA1-510A_Glucocorticoid_Receptor_Nr3c1_P04150-1_Rabbit.svg,antigenImageFileNamePDP:PA1-510A_Glucocorticoid_Receptor_Nr3c1_P04150-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325823

Product Specific Information

PA1-510A detects glucocorticoid receptor (GR) from human, rat, and reptile tissues. This product detects both the unactivated and activated forms of the receptor.

PA1-510A has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract.

PA1-510A immunizing peptide corresponds to amino acid residues 150- 175 from human GR.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.