Thermo Fisher Scientific LAG3 Recombinant Rabbit Monoclonal Antibody (BLR028F)
LAG3 단백질을 표적으로 하는 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP 등에 사용 가능. HEK293 세포에서 발현된 재조합 항체이며, 항원 친화 크로마토그래피로 정제됨. 인간 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR028F |
| Immunogen | Recombinant protein representing the extracellular domain of human Lymphocyte Activation Gene 3 (LAG3, residues 23–450) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4 °C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application Note:
- For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
LAG-3 is a 70 kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. It binds to MHC class II with higher affinity than CD4 and is involved in negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells. CD8⁺ T cells typically express higher levels than CD4⁺ T cells. Coexpression of LAG-3 and CD49b has been proposed as a marker for Type 1 regulatory T cells (Tr1 cells).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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