Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (5H10), PE-Texas Red

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5H10

Immunogen

Con A-stimulated BALB/c splenic T cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P01731-1,ncbiNodeId:10090,antigenRange:1-247,antigenLength:247,antigenImageFileName:MCD0817_CD8_P01731-1_Rat.svg,antigenImageFileNamePDP:MCD0817_CD8_P01731-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

View additional formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-Alexa Fluor 750
• FITC
• Pacific Blue
• Pacific Orange
• PE
• PE-Cyanine7
• PerCP
• Request custom conjugation

Excitation/Emission Max

567/615 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374589

Product Specific Information

The 5H10 monoclonal antibody (mAb) recognizes the mouse CD8a chain (Ly-2). Antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive T cells. The 5H10 mAb has been used in other labs for flow cytometry and inhibition of T cell responses to IL-21.

Each lot is tested by flow cytometry using freshly harvested mouse splenocytes. For PE-Texas Red conjugates: excitation 488 nm/peak emission 615 nm.

Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.