Thermo Fisher Scientific Phospho-Rb (Ser612) Polyclonal Antibody
Retinoblastoma 단백질의 Ser612 인산화 형태를 특이적으로 검출하는 rabbit polyclonal antibody. Western blot 및 Immunocytochemistry에 적합. Human, Mouse, Non-human primate 반응성. 고순도, 액상 형태, -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RB1 (Accession P06400), corresponding to amino acid residues around phosphorylated Ser612 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817196 |
Product Specific Information
Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Ser612.
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E.
Rb protein may act by regulating transcription, and loss of its function leads to uncontrolled cell growth.
Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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