Thermo Fisher Scientific MASP2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.04–0.4 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
PVCEPVCGLS ARTTGGRIYG GQKAKPGDFP WQVLILGGTT AAGALLYDNW VLTAAHAVYE QKHDASALDI RMGTLKRLSP HYTQAWSEAV FIHEGYTHDA GFDNDIALIK LNNKVVINSN ITPICLPRKE AESFMRTDDI GTASGWGL

• Highest antigen sequence identity to the following orthologs:
  • Mouse: 78%
  • Rat: 78%

Target Information

Mannose (or mannan)-binding lectin (MBL), also known as serum mannose-binding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain.
MBL is a key component in immune response, directly triggering neutralization of invading microorganisms by an antibody-independent mechanism. Mutations of human MBL are associated with immunodeficiency resulting from reduced ability to initiate complement fixation.

In humans, three types of MBL-associated serine proteases (MASP-1, MASP-2, MASP-3) and a truncated form of MASP-2 (small MBL-associated protein; sMAP or MAp19) complex with MBL to activate the lectin pathway of the complement system. Activated MASPs subsequently cleave and activate downstream components of the complement pathway.
MASP-3 is an alternatively spliced product from the MASP-1 gene and may function to inhibit MASP-2 by competing for MBL binding and inhibiting its activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.