Thermo Fisher Scientific 6x-His Tag Chimeric Recombinant Human Monoclonal Antibody (3D5)
6x-His 태그를 인식하는 인간 단클론 항체로, C-말단 및 내부 His-tag 단백질 검출에 적합. Western blot, ELISA, Flow cytometry 등 다양한 분석에 사용 가능. HEK293 세포 발현, Protein A 정제, 비결합형 액상 형태로 제공.
- 카탈로그번호
- MA551572
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Flow Cytometry (Flow): Assay-dependent
- ELISA (ELISA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Human / IgG2, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | Purified His tagged Fab fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Target Information
The 6x His tag is a synthetic oligo peptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either N- or C-terminal regions of recombinant proteins to enable isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins by fusing known epitopes, making it possible to identify proteins lacking specific antibodies. His-tagged proteins are purified using metal chelate chromatography due to the strong and selective affinity of histidine clusters for Ni²⁺ ions, typically on Ni²⁺-NTA adsorbents.
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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