Thermo Fisher Scientific F(Ab`)2 Biotin Polyclonal Antibody, PE
PE 형광 표지된 Biotin F(Ab`)2 다클론 항체로, Western blot, Flow cytometry, ELISA에 사용 가능. Goat 유래 IgG 폴리클론 항체이며, 높은 특이성과 안정성을 제공. 연구용으로만 사용되며, 장기 보관 시 -20°C 저장 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:100–1:250
Flow Cytometry (Flow)
- Tested Dilution: 1 µg/1×10⁶ cells
ELISA
- Tested Dilution: 1:100–1:250
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with deionized water (or equivalent).
- Stable at 4°C as undiluted liquid; dilute only prior to immediate use.
- Centrifuge if not completely clear after standing at room temperature.
- Do not freeze after reconstitution.
- Store reagent in the dark; use subdued lighting during handling and incubation of cells prior to analysis.
Assay Result:
Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, and Biotinylated IgG.
No reaction observed against anti-Pepsin or anti-Goat IgG F(c).
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7).
It is required by all organisms and synthesized only by bacteria, yeasts, molds, algae, and some plants.
Biotin participates in carbon dioxide incorporation into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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