Thermo Fisher Scientific E.coli Combined HCP Polyclonal Antibody, Biotin
E.coli 유래 Host Cell Protein 검출용 폴리클로날 항체로, Biotin 결합 형식으로 제공됩니다. Western blot, ELISA, Immunoprecipitation에 적합하며, Protein A로 정제된 고순도 항체입니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
- 카탈로그번호
- 200-406-M61
- 판매단위
- pk
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Thermo Fisher Scientific E.coli Combined HCP Polyclonal Antibody, Biotin
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000–1:2,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E. coli (BL21). |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is directed against high molecular weight and low molecular weight E. coli Host Cell Proteins.
The product was purified from monospecific antiserum by Protein A affinity chromatography.
Reactivity is directed against E. coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Target Information
Host Cell Proteins (HCPs) are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance.
HCP Antibodies are used to detect impurities in biologically-based therapeutics.
Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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