Thermo Fisher Scientific MARCO Monoclonal Antibody (PLK-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

0.6 µg/mL

-

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Functional Assay (Functional)

1:50

-

Product Specifications

Species Reactivity

Bovine, Human

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

PLK-1

Immunogen

Human alveolar macrophages (recognizes domain V between residues 420 and 431) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MARCO,uniProtId:Q9UEW3-1,ncbiNodeId:9606,antigenRange:420-431,antigenLength:520,antigenImageFileName:MA1-40315_MARCO_Q9UEW3-1_House_mouse.svg,antigenImageFileNamePDP:MA1-40315_MARCO_Q9UEW3-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2140582

Product Specific Information

Endotoxin: 0.40 EU/mg

Application Notes: IHC (frozen) - tissue sections can be fixed in acetone or 2% paraformaldehyde. Western blot - use non-reducing conditions. Flow - this antibody weakly stains alveolar macrophages by recognizing the extracellular domain of MARCO, with transfected COS cells used as a positive control. Immunoprecipitation - will immunoprecipitate MARCO as a 60 kDa and 50 kDa protein from transfected COS lysates. Functional Assays - blocks human alveolar macrophages by recognizing the extracellular doamin of MARCO.

Recommended positive control: Human alveolar macrophages; Negative control: All other human cells.

Target Information

MARCO, also known as SR-A6 or SCARA2, is a macrophage surface receptor and a member of the class A scavenger receptor family. As a part of the innate antimicrobial immune system, MARCO can bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. Due to its expression in tumor infiltrating macrophages, and its role in inhibiting immune responses within tumor environment, MARCO has been indicated as a potential target for cancer immunotherapy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.