Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), eBioscience
인간 CD4 단백질을 인식하는 OKT4 단클론 항체로, 주로 T-helper 세포 분석에 사용됩니다. Flow cytometry 및 immunoprecipitation에 적합하며, HIV 수용체 연구에 활용 가능합니다. 고순도 정제 및 4°C 보관 조건을 유지합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 4 |
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 1 µg/test | 58 |
| ELISA | - | 2 |
| Immunoprecipitation (IP) | Assay-Dependent | 2 |
| Neutralization (Neu) | - | 1 |
| Functional Assay | - | 8 |
| In vitro Assay | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT4 (OKT-4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467074 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Custom conjugation available upon request
Product Specific Information
Description:
The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by most thymocytes, a subpopulation of mature T cells (T-helper cells), and at low levels on monocytes. CD4 acts as a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these do not cross-block each other’s binding.
Applications Reported:
Flow cytometric analysis and immunoprecipitation.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage ≤1 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD4 antigen participates in the recognition of MHC class II molecules and functions as a co-receptor for HIV. It is primarily expressed on T-helper lymphocytes, and to a lesser extent on monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues such as brain and gut. CD4 contributes to early T-cell activation via interaction with the TCR complex and Lck kinase. It may also mediate neuronal damage in infectious or immune-related CNS disorders. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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