Thermo Fisher Scientific V-ATPase A Polyclonal Antibody
Rabbit 다클론 항체로 Human, Mouse, Rat의 V-ATPase A 단백질 검출에 적합. Western blot과 ELISA에 사용 가능. 합성 펩타이드(AA 500-600) 면역원 사용. 액상 형태로 -20°C 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 500–600 of human ATP6V1A (NP_0016812) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.82 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805881 |
Product Specific Information
Immunogen sequence:
SLAETDKITL EVAKLIKDDF LQQNGYTPYD RFCPFYKTVG MLSNMIAFYD MARRAVETTA QSDNKITWSI IREHMGDILY KLSSMKFKDP LKDGEAKIKS D
Positive Samples:
293T, LO2, HeLa, Mouse brain, Mouse heart, Mouse kidney
Target Information
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase-dependent organelle acidification is essential for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
V-ATPase consists of:
- V1 domain (cytosolic): three A and three B subunits, two G subunits, and C, D, E, F, and H subunits. Contains the ATP catalytic site.
- V0 domain (transmembrane): five different subunits (a, c, c′, c″, d).
Multiple isoforms and alternatively spliced transcript variants exist. This encoded protein is one of two V1 domain A subunit isoforms and is expressed in all tissues. Transcript variants derived from alternative polyadenylation exist.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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