Thermo Fisher Scientific Phospho-JNK1/JNK2/JNK3 (Tyr185) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phosphorylated JNK1/2/3 (Tyr185). Suitable for WB, IHC(P), and ICC/IF. Detects endogenous phosphorylated JNK forms. Supplied as liquid, 1 mg/mL, stored at -20°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAPK8 (Accession P45983), corresponding to amino acid residues around phosphorylated Tyr185. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816380 |
Product Specific Information
This antibody detects endogenous levels of JNK1/2/3 only when phosphorylated at Tyrosine 185.
Target Information
C-Jun N-terminal Kinase (JNK), also known as Mitogen-activated protein kinase (MAPK8), belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are serine-threonine protein kinases activated by various extracellular stimuli and mediate signal transduction from the cell surface to the nucleus. JNK participates in cellular processes such as proliferation, differentiation, transcription regulation, and development. JNK pathways are activated by stress and inflammatory signals and are expressed as ten isoforms due to alternative mRNA splicing, with JNK1 and JNK2 being predominant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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