
Thermo Fisher Scientific Complement C4d Polyclonal Antibody
Human C4d 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P), IHC(F), ICC/IF에 사용 가능. 열 유도 항원 회복(Tris/EDTA, pH 8.5) 권장. Protein G 정제 및 PBS 기반 보존액에 0.1% ProClin 950 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:80 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20–1:80 |
| Immunocytochemistry (ICC/IF) | 1:20–1:80 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1241–1256 of C4 conjugated to Keyhole limpet Haemocyanin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% ProClin 950 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2066857 |
Product Specific Information
- Heat-mediated antigen retrieval using Tris/EDTA (pH 8.5) is recommended for the staining of paraffin sections.
- Rabbit anti-Human C4d antibody is specific for human complement split product C4d.
Target Information
C4 is a component of the classical complement pathway and is split by activated C1s into C4a and C4b. Together, C2a and C4b form the classical C3 convertase.
The molecular mass of C4 is approximately 204 kDa and it consists of three subunits: α (97 kDa), β (75 kDa), and γ (33 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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