Thermo Fisher Scientific MIF Recombinant Rabbit Monoclonal Antibody (BLR141J)
인간 MIF 단백질(2-115)을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. 고순도 정제되어 있으며 PBS 버퍼에 0.2% BSA 및 0.09% sodium azide를 포함합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR141J |
| Immunogen | Recombinant protein representing Human Macrophage Migration Inhibitory Factor (residues 2–115) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
MIF (Macrophage Migration Inhibitory Factor) was one of the first cytokine activities discovered and initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989).
Recently, MIF was rediscovered as a pituitary hormone acting as a counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995).
MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998).
MIF plays a critical role in host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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