Thermo Fisher Scientific Mouse IL-7R alpha/CD127 (soluble) Fc Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

CHO cells

Amino acid sequence

ESGNAQDGDL EDADADDHSF WCHSQLEVDG SQHLLTCAFN DSDINTANLE FQICGALLRV KCLTLNKLQD IYFIKTSEFL LIGSSNICVK LGQKNLTCKN MAINTIVKAE APSDLKVVYR KEANDFLVTF NAPHLKKKYL KKVKHDVAYR PARGESNWTH VSLFHTRTTI PQRKLRPKAM YEIKVRSIPH NDYFKGFWSE WSPSSTFETP EPKNQGGWDG GPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

Tag

Fc-tag

Molecular weight

51 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to bind immobilized Murine IL-7 in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Mouse IL-7R alpha/CD127 (soluble) Fc is a 51.0 kDa protein containing 452 amino acid residues consisting of the extracellular domain of the mature sequence, as well as a 230 C-terminal Fc region linked by two glycines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.