Thermo Fisher Scientific CD146 Monoclonal Antibody (P1H12), FITC, eBioscience
CD146 단백질을 인식하는 P1H12 단일클론 항체로, FITC 형광 표지되어 있습니다. 인간 및 다양한 종에 반응하며, 유세포분석(Flow Cytometry)과 면역형광(ICC/IF)에 적합합니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD146 Monoclonal Antibody (P1H12), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 17 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rabbit |
| Published Species | Dog, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P1H12 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043806 |
Additional Formats
- Unconjugated
- eFluor 450
- eFluor 660
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Yellow 730
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The monoclonal antibody P1H12 recognizes CD146, also known as MUC18, s-endo, Endo-CAM, Mel-CAM, a member of the Ig superfamily of proteins.
CD146 is expressed on endothelial cells, bone marrow fibroblasts, and some tumors (especially melanoma). It is also found in mesenchymal and endometrial stromal cells.
CD146 expression on circulating blood cells is limited to a subset of T cells rather than endothelial cells. The protein is heavily glycosylated, with more than 50% of its mass from carbohydrates.
- Cross-reactivity: Mouse, Rabbit, Dog (not Rat)
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of human peripheral blood cells and HUVEC
- Recommended Usage: 5 µL (0.25 µg) per test (in 100 µL final volume)
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD146 (MCAM) is an integral membrane glycoprotein of the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and various normal and malignant cell types.
CD146 functions as a calcium-independent cell adhesion molecule and may participate in signal transduction via Fyn kinase, influencing actin cytoskeleton assembly.
It plays a role in endothelial cohesion and may facilitate melanoma cell interaction with vascular tissue, promoting tumor spread.
CD146 also acts as a surface receptor triggering tyrosine phosphorylation of FYN and PTK2 and transient increases in intracellular calcium concentration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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