Thermo Fisher Scientific IL8 Monoclonal Antibody (4F10)
인간 IL-8 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot과 ELISA에 적합합니다. 친화 크로마토그래피로 정제된 액상 형태이며, 보존제 무첨가 PBS 용액에 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F10 |
| Immunogen | IL8 (AAH13615.1, 21 a.a. ~ 99 a.a) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor belonging to the CXC subfamily of chemokines. It binds to cell surface receptors IL-8RA and IL-8RB and functions as a chemoattractant and potent angiogenic factor. IL-8 expression and secretion can be induced by various inflammatory stimuli in macrophages and endothelial cells, where it is stored in Weibel-Palade bodies.
IL-8 was first isolated from osteosarcoma cells and contains the ELR motif (N-terminal Glu-Leu-Arg sequence), signaling through CXCR1 and CXCR2 receptors. Previous names include neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP), and protein 3-10C. IL-8 and other CXC chemokines form a gene cluster on chromosome 4q. Studies show IL-8 plays roles in angiogenesis, tumor growth, and bronchiolitis pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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