Thermo Fisher Scientific ERO1L Recombinant Superclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 2 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Pig, and Bovine.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They consist of multiple different recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, and ensuring lot-to-lot consistency.
While functionally similar to polyclonal antibodies—recognizing multiple epitopes and providing higher sensitivity for low-abundance targets—Superclonal™ antibodies have a defined mixture of light and heavy chains that can be reproduced identically in every lot.
Note: Formerly called “Recombinant polyclonal antibody”, now rebranded as “Recombinant Superclonal™ antibody”. Physical product and performance remain unchanged.

Target Information

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in the ER membrane as a peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for ER retention and couples oxygen reduction to de novo disulfide formation.
ERO1L is broadly distributed at low levels across tissues, with highest expression in the upper digestive tract, and is stimulated by hypoxia via HIF-signaling. It can exist as monomer or homodimer and interacts with PDILT.
After translation, ERO1L undergoes N-glycosylation, and its disulfide bonds form the redox-active center. The Cys-94/Cys-99 disulfide accepts electrons from P4HB and transfers them to the active site disulfide Cys-394/Cys-397.
ERO1L oxidizes P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44. It plays roles in immunoglobulin folding, oxidative stress, cholera toxin release, and ER stress-induced CHOP-dependent apoptosis through IP3R1 activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.