
Thermo Fisher Scientific Ly-6A/E Monoclonal Antibody (D7)
Ly-6A/E (Sca-1) 단백질을 인식하는 Rat IgG2a 단클론 항체로, Mouse 시료에서 Western blot, Flow cytometry, Immunoprecipitation 등에 사용 가능. 0.5 mg/mL 농도의 액상 형태이며 Protein G로 정제됨. 줄기세포 표지자인 Sca-1 연구에 적합.
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Thermo Fisher Scientific Ly-6A/E Monoclonal Antibody (D7)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Flow Cytometry (Flow) | 1 µg/1 million cells | View 14 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bovine, Mouse |
| Host/Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D7 |
| Immunogen | IL-2-dependent mouse T-cell line CTL-L |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077516 |
Available Conjugated Formats
Product Specific Information
MA1-70082 detects Ly-6A/E in mouse samples.
It has been successfully used in Western blot, flow cytometry, and immunoprecipitation procedures.
The immunogen is IL-2-dependent mouse T-cell line CTL-L.
Target Information
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family.
It is expressed on multipotent hematopoietic stem cells (HSC) and serves as a marker for HSC in mice.
Sca-1 is used with lineage depletion antibodies to identify murine HSC and is found in fetal liver, adult bone marrow, peripheral blood, and spleen.
It may play a role in regulating B and T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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