Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), Biotin
DYKDDDDK(FALG) 태그 인식용 Biotin 결합 단클론 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합합니다. 마우스 IgG2a, kappa 아이소타입이며, FLAG 태그 단백질 검출 및 정량에 유용합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29E4.G7 |
| Immunogen | Synthetic peptide corresponding to FLAG™ epitope tag DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Glycine and cysteine residues were added to termini for coupling. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Agarose
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not completely clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
This antibody is directed against the FLAG™ epitope tag and is useful for detecting FLAG-tagged proteins in various assays. It recognizes the FLAG™ epitope (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino or carboxy termini of target proteins.
Target Information
DYKDDDDK (FLAG) tag is an eight amino acid peptide used as a protein tag for purification, detection, and localization. It can be fused to N- or C-terminus of proteins, enabling identification and isolation using anti-FLAG antibodies. The FLAG tag is hydrophilic and less likely to denature or inactivate proteins compared to other tags. Widely used in molecular biology and biochemistry research for protein-protein interaction, expression, and localization studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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