Thermo Fisher Scientific Themis Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Themis. Recombinant protein control fragment (Product #RP-94952). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Themis,uniProtId:Q8N1K5-1,ncbiNodeId:9606,antigenRange:68-163,antigenLength:641,antigenImageFileName:PA5-56740_Themis_Q8N1K5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-56740_Themis_Q8N1K5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2648436

Product Specific Information

Immunogen sequence: EGCESLQPFE LPMNFPGLFK IVADKTPYLT MEEITRTIHI GPSRLGHPCF YHQKDIKLEN LIIKQGEQIM LNSVEEIDGE IMVSCAVARN HQTHSF

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 79%.

Target Information

Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.