Thermo Fisher Scientific TNF alpha Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10596988

Product Specific Information

Description: BMS175 is a polyclonal Antibody thatreacts with rat TNF-alpha.

Applications Tested: ELISA, Functional Studies (Blocking), Western Blotting.

Purity: >99%.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.