Norgen Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit

Norgen Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit

SKU: 58600
All-in-one system for the purification of exosomes and subsequent isolation of RNA from plasma/serum samples.

Features and Benefits

• Purification and enrichment of intact plasma/serum exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile plasma/serum input volume (4–10 mL)
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No ultracentrifugation, filtration, or special syringes required
• No precipitation reagents nor overnight incubation needed
• Compatible with plasma/serum from most species
• Pure exosomes free from RNA-binding proteins
• Spin column chromatography using Norgen’s proprietary resin
• Purified RNA suitable for downstream applications including Small RNA Sequencing, RT-PCR, Northern blot, and expression arrays

Kit Variants

Supporting Data

Figure 1. Isolation of RNA from exosomes purified from different plasma volumes (Mini Kit).
2 μL of the isolated RNA was used in RT-qPCR to assess amplification of plasma exosomal miR-26a.

A. Avg. Ct. Value of Plasma Exosomal miR-26a

B. Relative Amount of Plasma Exosomal miR-26a

Figure 2. Comparing exosome yields from different purification methods (Mini Kit).

A. Concentration (×10¹³/mL Plasma)

B. Particle Size Distribution (nm)
Key data points: 55, 65, 75, 185
Legend: Competitor L, Competitor S, Norgen

Figure 3. NanoSight data showing intact exosomes purified from 1 mL and 10 mL plasma.
Text extracted:
Plasma - 1 mL Input Plasma - 10 mL Input

Figure 4. Isolation of RNA from exosomes purified from different plasma volumes (Midi Kit).

A. Avg. Ct. Value of Plasma Exosomal miR-26a

B. Relative Amount of Plasma Exosomal miR-26a

Figure 5. Isolation of RNA from exosomes purified from different plasma volumes (Maxi Kit).

A. Avg. Ct. Value of Plasma Exosomal miR-26a

B. Relative Amount of Plasma Exosomal miR-26a

Kit Specifications

*Refer to product insert for average yields and quantification methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature.
Stable for 2 years after shipment.
Warm Lysis Buffer A for 20 minutes at 60°C if salt precipitation is observed.

Important Note

Suitable for exosome purification from fresh or frozen serum/plasma collected on EDTA or Citrate.
Do not use plasma from heparin-treated blood, as heparin interferes with downstream applications such as RT-PCR.

Kit Components

Documentation

• Protocol - Maxi Kit (15 prep)
• Application Note - Small RNA Sequencing of Plasma Exosomes
• Safety Data Sheet - Maxi Kit

FAQs (Selected)

• Centrifugation should be at room temperature; 4°C does not affect performance.
• Adding incorrect reagent volumes can reduce yield and quality.
• Second elution is possible; use half volume and separate tube.
• Exosomes contain little RNA; increasing input volume can raise yield.
• Store purified exosomes at −80°C; avoid freeze–thaw cycles.

Citations

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