Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (B-A15), FITC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B-A15

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

10 µL/Test

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.2-7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598062

Product Specific Information

Description: BMS127 recognizes natural and recombinant human IL1 beta.

IL-1beta is a pro-inflamatory cytokine and is expressed by many cells including macrophage, NK cells, monocytes, and neutrophils. It belongs to the IL-1 family cluster. Associated activities with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption.

Applications Tested: Flow Cytometry (Intracellular Staining).

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.