Thermo Fisher Scientific Neurofilament (NF-L) Recombinant Rabbit Monoclonal Antibody (NEFL, 2983R)
신경세포에서 NF-L 단백질을 특이적으로 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. IHC(P) 검증 완료, 다양한 종에 반응. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태. 신경섬유 연구용에 적합.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution
- 0.25–0.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | NEFL, 2983R |
| Immunogen | Recombinant full-length human NEFL protein (UniProt ID: P07196-1, amino acids 1–543) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons and are composed predominantly of three major proteins: NF-L, NF-M, and NF-H.
NF-L (Neurofilament Light) is the light or low molecular weight subunit (~70 kDa) and plays a crucial role in maintaining neuronal caliber.
NF-M and NF-H represent the medium and heavy subunits, respectively, with NF-H typically migrating at 180–220 kDa depending on the species.
Expression of these proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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