Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), APC-eFluor 780, eBioscience

Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), APC-eFluor 780, eBioscience™

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, Biotin
• Brilliant UV 395, 563, 661, 805
• Brilliant Violet 421, 650, 711
• eFluor 450, 506, 570, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor belonging to the EGF-TM7 family. It contains seven EGF-like domains, seven transmembrane regions, and a cytoplasmic domain homologous to TM7 superfamily members. F4/80 is expressed by most mature macrophages and serves as a key marker for this cell population. It may also be expressed by eosinophils, Langerhans cells, and some dendritic cells. Expression begins during early myeloid development and can be upregulated by M-CSF stimulation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse resident peritoneal exudate cells at ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorophore Information:
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Ensure instrument compatibility.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal loss of brightness.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein in the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies during macrophage maturation and activation. It is found on macrophages in tissues such as liver (Kupffer cells), skin (Langerhans), brain (microglia), gut, peritoneum, lung, thymus, bone marrow, and spleen red pulp. It is absent from macrophages in T cell zones of spleen and lymph nodes. F4/80 may play a role in generating efferent CD8⁺ regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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