
Thermo Fisher Scientific CD22 Monoclonal Antibody (RFB-4), FITC
인간 CD22를 인식하는 Mouse IgG1 단클론 항체로 FITC 형광 표지. Flow Cytometry 및 Immunocytochemistry에 사용 가능. 높은 형광 효율과 수용성, 안정적인 신호 제공. 4°C 암소 보관 권장.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: -
- Publications: View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: Assay-Dependent
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RFB-4 |
| Immunogen | Human CD22 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | PBS with 4 mg/mL BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10373401 |
Additional Formats
Product Specific Information
FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
Target Information
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130–140 kDa.
It is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell differentiation at approximately the same stage as CD19.
Membrane expression is limited to mature B cells (CD22+) and absent in plasma cells (CD22−), useful for phenotyping mature leukemias.
CD22 is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
It preferentially binds to alpha2,6-linked sialic acid and participates in regulation of B-cell receptor signaling through interactions with Src family tyrosine kinases and phosphatases.
Analyte Specific Reagent
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