Thermo Fisher Scientific CD19 (B-Lymphocyte Marker) Monoclonal Antibody (CD19/3116)
CD19 단백질을 표적으로 하는 인간 반응성 mouse monoclonal 항체. IHC, Flow cytometry, ELISA 등 다양한 응용에 적합. Protein A/G 정제, PBS 기반 buffer에 0.05% BSA 및 sodium azide 포함. 4°C 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | Assay-dependent |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD19/3116 |
| Immunogen | Recombinant fragment of human CD19 protein (around aa96–281) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells defined by kappa or lambda light chain expression. CD19 can also be found on certain myeloid leukemia cells, particularly of monocytic lineage. It serves as an early and broad B-cell–restricted antigen and functions as a key regulator of normal and malignant B cell proliferation.
CD19 acts as a coreceptor with the B cell receptor (BCR), lowering the threshold for antigen receptor-dependent stimulation. It can also signal independently of BCR co-ligation, serving as a central regulatory hub for multiple signaling pathways. Mutation of the CD19 gene can lead to hypogammaglobulinemia, while overexpression results in B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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