Thermo Fisher Scientific CD74 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1).
Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons: exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and cytoplasmic tail.
DRA does not have polymorphisms in the peptide-binding region and serves as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5. Within the DR molecule, the beta chain contains all polymorphisms specifying peptide-binding specificities. Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation.
HLA-DR is expressed primarily on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes. The DR, DQ, and DP loci encode the major expressed products of the human class II region. Human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and play a critical role in initiating the immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.