Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP
Thermo Fisher Scientific의 Donkey anti-Sheep IgG (H+L) AP-conjugated 항체는 고도로 교차 흡착된 2차 항체로 Western blot, IHC, ELISA에 적합합니다. 높은 특이성과 감도를 제공하며, 인간·마우스·토끼 IgG에 대한 교차 반응이 최소화되었습니다. 연구용으로 사용됩니다.
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Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534724 |
| Target | IgG |
| Cross Adsorption | Against human, mouse, and rabbit IgG |
| Antibody Form | Whole Antibody |
Additional Formats
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins. They are useful in the detection, sorting, or purification of their specified targets. Secondary antibodies offer increased versatility, enabling users to employ various detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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