Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC
FITC 형광 표지된 Donkey anti-Sheep IgG (H+L) 2차 항체로 고도의 교차흡착 처리되어 높은 특이성과 낮은 비특이 결합을 제공합니다. ICC/IF 및 유세포분석에 적합하며, 498/517 nm에서 최적 형광 반응을 나타냅니다. 연구용으로 사용됩니다.
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- A16049
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Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Sheep |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534722 |
| Target | IgG |
| Cross Adsorption | Against human, mouse and rabbit IgG |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of their specified targets. Secondary antibodies offer increased versatility enabling users to use various detection systems (e.g. HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g. immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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